Search results for " IgG"
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Efficient concentration of a low-titer bovine IgG with high recovery in low volume
The described process enabled concentration of a bovine IgG antibody from 50.0 to 0.165 L in less than 5 h.
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Antibody Production in Microbial Hosts
Aglycosylated antibodies as alternate therapeutic immunoglobulins
Antibodies constitute an important class of serum glycoproteins, and the IgG isotype is the most common isotype used for pharm…
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USP Publishes Monoclonal Antibody Guidelines
General Chapter provides analytical procedures for murine, chimeric, and humanized IgG isotype mAbs and subtypes (e.g., IgG1 and IgG2). Because subclasses show differences in amino acid sequence and …
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N-Glycan Analysis of Biotherapeutic Proteins
In addition, some IgGs such as Erbitux (cetuximab) are also N-glycosylated within the fragment antigen-binding (Fab) region. Figure 1A represents some of the therapeutic effects of biantennary N-glyca…
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From Darwin to Recombinant Fc Multimers
CSL was interested to test the approach of topical application of immunoglobulins into the lungs and performed initial feasibility assessment studies of nebulization of IgG. The conclusion was that ne…
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Single-Domain Antibodies for Brain Targeting
Intact antibodies (immunoglobulins IgG, IgM, IgA, and IgE) are highly specific targeting reagents that form part of our key defence against pathogens and toxins. IgG, the main serum antibody, the inta…
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Implications of Cell Culture Conditions on Protein Glycosylation
Multi-chain proteins, such as IgG antibodies, additionally have a quaternary structure resulting from structural associations between the subunits.
The choice of host-cell line for recombinant pro…
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Advances in Engineering of Protein-Based APIs
“Ongoing IgG-based modular domain engineering and platform development in multispecifics present great potential to elicit synergistic activities, enhanced therapeutic index (efficacy, selectivity, no…
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Witnessing Major Growth in Next-Generation Antibodies
“The development of next-generation antibodies typically involves more uncertainty, complexity, and risk than well-established IgG (immunoglobulin G) technology on both the clinical side and with resp…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Purification of these Ab fragments utilizes normal chromatographic procedures unless the H-chain is from the VH3 IgG gene family in which case it will bind to protein A. After purification these small…